Shanghai Xinfan Bio: Thermo Fisher launches a new generation of membrane protein extraction products

Membrane Protein

Membrane proteins play a crucial role in various cellular functions, including signal transduction, material transport, and intercellular communication. These proteins are also among the most targeted drug candidates in the pharmaceutical industry, with approximately two-thirds of therapeutic molecules interacting with one or more membrane proteins. Understanding the crystal structure of these proteins is essential for revealing their functional mechanisms and how they interact with drugs. However, despite their high abundance in the cellular proteome (20–30%), only about 0.1% of their structures have been determined to date.

Where is the bottleneck?

The main challenge lies in the lack of efficient reagents and protocols for extracting sufficient quantities of membrane proteins from different cell types. While several methods exist for isolating membrane proteins from eukaryotic cells and tissues—such as subcellular fractionation using sucrose density gradients, Triton X-114 detergent extraction, and high salt or pH buffer treatments—most of them are time-consuming, labor-intensive, and require expensive equipment like ultracentrifuges.

Thermo Fisher

Previously launched by Pierce, the Mem-PER kit is based on the Triton X-114 method but involves a complicated procedure. The hydrophobic fragments extracted often need further processing for downstream applications. To address this, Pierce recently introduced a new generation of membrane protein extraction kit — the Mem-PER Plus kit. This improved version simplifies the process of enriching intrinsic and membrane-associated proteins, requiring only a standard bench-top centrifuge.

Integral membrane proteins differ from peripheral ones in terms of their binding strength to the membrane. Intrinsic membrane proteins contain one or two transmembrane domains, which require effective solubilization and separation from intracellular proteins. The Mem-PER Plus kit is suitable for both mammalian cells and hard or soft tissues. Compared to previous versions, it offers greater simplicity and compatibility with a wide range of downstream applications, such as SDS-PAGE, Western blot, BCA assays, and immunoprecipitation.

The Mem-PER Plus kit uses a mild detergent to selectively extract intrinsic and membrane-associated proteins. This selective approach avoids the complexities of traditional hydrophobic phase separation, leading to better reproducibility and higher throughput.

The process begins with lysing the cells using a mild detergent to release soluble proteins, followed by a second detergent to dissolve the membrane proteins. The entire procedure can be completed in under an hour, without the need for specialized equipment—only a bench-top centrifuge, homogenizer, and pipette are required. While extraction efficiency and yield may vary depending on the cell type and the number of transmembrane domains, studies have shown that the extraction efficiency for proteins with one or two transmembrane regions can reach up to 90%, with intracellular protein contamination typically below 10%.

Elisa Kit

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Shanghai

Elisa

Experimental survey

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Experimental generation

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Immunohistochemistry experiment

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Free test

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Shanghai Xinfan Biological

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