In the lead-up to the winter break, many school laboratories face challenges in maintaining the cleanliness of precision glass equipment. Due to a lack of proper cleaning protocols and insufficient personnel dedicated to this task, daily cleaning becomes excessive. As a result, common glassware used for ELISA kits often fails to function properly. Instruments may overheat or fail to maintain the required temperature for experiments, which can pose serious safety risks to students if not addressed promptly. In some cases, samples have to be discarded, and the entire experiment may be compromised due to unclean equipment.
With the rapid development of science and technology in the 21st century, advanced laboratory equipment such as low-temperature coolant circulation pumps, low-temperature thermostats, high-precision temperature control units, constant temperature water baths, fuel tanks, high-temperature thermostats, ultrasonic cell disruptors, snowflake ice machines, freeze dryers, chromatography lab freezers, double-layer glass reactors, photochemical reaction systems, circulating water pumps, and water baths have been increasingly adopted in ELISA labs. However, these high-tech devices require regular cleaning; otherwise, they can become hidden hazards that affect student health and experimental accuracy.
Currently, the cleaning process in most labs involves two main approaches: either contacting manufacturers for professional cleaning services or purchasing cleaning agents like soap, detergents, washing powders, liquid solutions, and organic solvents. However, without careful selection and proper techniques, it is difficult to achieve effective results. For example, in July, some precision instruments at a university in Nanjing were cleaned using improper methods, leading to damage and reduced performance.
Here are some standard cleaning procedures for different types of laboratory glassware:
1. **General Glass Equipment** (e.g., flasks, beakers): Start by rinsing with tap water, then use soap or detergent and a brush to scrub the inside. Rinse thoroughly with tap water, followed by three rinses with purified water. Shake the container along the walls to ensure complete removal of residue.
2. **Measuring Instruments** (e.g., burettes, pipettes, volumetric flasks): Clean with soap or detergent but avoid using brushes to prevent scratches on sensitive surfaces.
3. **Precision or Difficult-to-Clean Glassware** (e.g., burettes, pipette tips, volumetric flasks, colorimetric tubes, glass fritted funnels): First rinse with tap water, drain, and soak in a chromic acid cleaning solution for a period of time. Then rinse with tap water and purify water three times.
4. **Hand Washing**: Always wash hands before handling equipment to avoid transferring oils or contaminants onto the glassware, which can increase cleaning difficulty.
5. **Drying and Storage**: A clean glass instrument should not retain water droplets when inverted. It can be air-dried on a clean rack, or dried using a warm air dryer at 60–70°C. However, measuring instruments should never be baked. When storing, place them in a safe, dry location, using paper pads to prevent scratches and ensure long-term preservation.
Proper maintenance and cleaning of laboratory equipment are essential for both safety and experimental success. Following established guidelines helps prevent accidents and ensures accurate results. Please take these practices seriously to protect yourself and others in the lab.
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