Follicle stimulating hormone (FS) ELISA kit began to do activities
Follicle Stimulating Hormone (FS) ELISA Kit
Basic Information
Follicle Stimulating Hormone (FSH), along with Luteinizing Hormone (LH), is classified as a gonadotropin. These hormones play a crucial role in the maturation of ovarian follicles and the stimulation of estrogen production. FSH is essential for regulating the menstrual cycle and promoting ovulation in females, while in males, it supports spermatogenesis.
Follicle Stimulating Hormone (FS) ELISA Kit
Introduction to Inspection Technology
Double Antibody Sandwich Method
1. Coating: The primary antibody is diluted to a concentration of 1–10 μg/ml using 0.05 M phosphate-buffered saline (PBS) at pH 9.6. A volume of 0.1 ml is added to each well of a polystyrene microtiter plate and incubated overnight at 4°C. The solution is then discarded, and the plate is washed three times with washing buffer (each wash lasting 3 minutes).
2. Sample Addition: 0.1 ml of the sample to be tested is added to each coated well. The plate is incubated at 37°C for 1 hour. Simultaneously, blank wells, negative control wells, and positive control wells are also processed.
3. Enzyme-Labeled Antibody Addition: After washing, 0.1 ml of freshly prepared enzyme-conjugated secondary antibody (diluted according to titration) is added to each well. The plate is incubated at 37°C for 0.5–1 hour, followed by another wash.
4. Substrate Addition: 0.1 ml of TMB (3,3',5,5'-tetramethylbenzidine) substrate solution is added to each well and incubated at 37°C for 10–30 minutes to develop the color.
5. Reaction Termination: 0.05 ml of 2M sulfuric acid is added to each well to stop the reaction.
6. Result Interpretation: The results can be visually assessed on a white background. The darker the color, the stronger the positive signal. A "positive" result is indicated by a visible color change, while a "negative" result appears colorless or very pale. For more precise quantification, an ELISA reader is used to measure the optical density (OD) at 450 nm (or 410 nm if ABTS is used). If the OD value exceeds 2.1 times that of the negative control, the result is considered positive.
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A single-phase VFD, also known as a variable frequency drive, is a specialized electronic device used for precise control and regulation of single-phase motors. Unlike three-phase motors that are commonly used in industrial applications, single-phase motors are predominantly found in residential and small-scale applications.
The primary function of a single-phase VFD is to control the frequency and voltage supplied to the single-phase motor, thereby enabling accurate regulation of motor speed. By adjusting the frequency and voltage output, the VFD allows for smooth and precise control over the motor's rotational speed. This feature is particularly useful in applications where speed control is required, such as in residential HVAC systems, small-scale machinery, and household appliances.
Energy efficiency is a significant advantage offered by single-phase VFDs. By adjusting the motor speed to match the load requirements, the VFD reduces energy wastage and improves overall energy efficiency. When the motor operates at a lower speed during periods of low demand, energy consumption is significantly reduced, resulting in energy savings and lower operating costs.
Motor protection is another important aspect addressed by single-phase VFDs. They incorporate various protective features, including overload protection, short circuit detection, and thermal protection, which help safeguard the motor against damage due to excessive current, voltage fluctuations, or overheating. This ensures reliable motor operation, prolongs the motor's lifespan, and reduces the risk of unexpected failures.
Harmonic filtering is also a critical consideration in single-phase VFD applications. When single-phase VFDs operate, they can introduce harmonics into the power supply, which may cause issues such as voltage distortions and interference with other electrical equipment. To mitigate these problems, single-phase VFDs often incorporate harmonic filtering techniques to suppress harmonics and ensure a clean and stable power supply, maintaining power quality and preventing damage to connected equipment.
Control algorithms play a significant role in single-phase VFD operation. These algorithms allow for precise control and adjustment of motor speed, ensuring smooth acceleration, deceleration, and accurate speed regulation. Advanced control algorithms enable efficient motor operation and enhance overall system performance.
In summary, single-phase VFDs provide precise control and regulation of single-phase motors in residential and small-scale applications. With their energy efficiency, motor protection features, harmonic filtering capabilities, and advanced control algorithms, single-phase VFDs enhance motor performance, reduce energy consumption, and ensure reliable operation in various residential and small-scale applications.
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